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Easy two-hybrid library construction enriched for longer inserts. steps required in traditional two-hybrid library manufacturing and screening. Matchmaker Gold Yeast Two-Hybrid System. Four reporters and three promoters lead to fewer false positives; Interacting fusion proteins produce resistance to Here we describe how to use the Matchmaker GAL4-based yeast two-hybrid system to detect the interaction of the Agrobacterium type VI secretion system (T6SS)

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